Fr | Ar

Diane ANTONIOS GHOLAM

Enseignant Cadré - Professeur associé - D Pharm, MS, PhD, ERT.

Faculty of Pharmacy (FP)
Toxicology Laboratory (LABTOX)

(+961) 1 421 000 ext 2168 diane.antonios@usj.edu.lb

Dr Diane Antonios-Gholam is an Associate Professor of Toxicology specialized in immunotoxicology clinical analytical toxicology at the USJ school of Pharmacy. 

Dr Antonios-Gholam earned her D. Pharm in 2005 from USJ her PhD in 2009 jointly from both Paris Saclay (formerly Paris XI) and USJ. In 2022, she was registered in the European Register of Toxicologists (ERT), which comprises a list of toxicologists excelling in high standards of education, skills, experience, and professional standing. She is actively involved in several research projects including drug hypersensitivity reactions, clinical and occupational toxicology, fundamental and applied immunology and vaccination. She has presented her research at national and international conferences and has numerous peer-reviewed publications, showcasing her contributions to the fields of toxicology and immunology


• European registered Toxicologist (ERT)
• Doctorate in Pharmaceutical and Biological Sciences
• PhD in Cellular and Molecular Pathophysiology (applied to Immunotoxicology)
• MS in Toxicology
• Doctorate in Pharmacy (D.Pharm.)
• Master’s in Biomedical sciences

Medecine and Health

Research projects : drug hypersensitivity reactions, clinical occupational toxicology, fundamental applied immunology, vaccination, signaling pathways.

Grants received from USJ-Research Council and Programme CEDRE.

Peer reviewed original papers : 1-Heavy metal contamination in L. ebanese lettuce: Quantification health risk assessment. K Baassiri, D Antonios, A Milane. Journal of Food Composition Analysis. Volume 137, Part A, January 2025, 106871 2. Drug-induced hypersensitivity reactions in a Lebanese outpatient population: A decade-long retrospective analysis (2012-2021). J.Dagher, D.Antonios,S. Chollet-Martin, L.De Chaise Martin, M. Pallardy, H. Azouri, C. Irani . J Allergy Clin Immunol Glob. 2023 Sep 173(1):100169. 3. Assessment of work-related symptoms, perceived knowledge, and attitude among nail salon technicians. Lteif M, El Hayek MS, Azouri H, Antonios D. Toxicol Ind Health. 2020 Nov36(11):852-862. 4. Knowledge and Attitude Among Lebanese Women Toward Hazardous Chemicals Used in Nail Cosmetics. Lteif M, Samia El Hayek M, Azouri H, Antonios D. J Community Health. 2020 Oct;45(5):922-931. 5. Vaccine coverage for Lebanese citizens and Syrian refugees in Lebanon. M Kmeid, H Azouri, R Aaraj, E Bechara, D Antonios Int Health. 2019 Nov 13;11(6):568-579. doi: 10.1093/inthealth/ihz023. 6. IL-27 production and regulation in human dendritic cells treated with the chemical sensitizer NiSO4. R Bechara, M Nabhan, D Antonios, H Azouri, M Pallardy. Chem Res Toxicol. 2018 Nov 27 7. Nickel Sulfate Promotes IL-17A Producing CD4+ T Cells by an IL-23-Dependent Mechanism Regulated by TLR4 and Jak-STAT Pathways. Bechara R, Antonios D, Azouri H, Pallardy M. J Invest Dermatol. 2017 Oct;137(10):2140-2148 8. An Evaluation of the Stemness, Paracrine, and Tumorigenic Characteristics of Highly Expanded, Minimally Passaged Adipose-Derived Stem Cells. El Atat O, Antonios D, Hilal G, Hokayem N, Abou-Ghoch J, Hashim H, Serhal R, Hebbo C, Moussa M, Alaaeddine N. PLoS One. 2016 Sep 15;11(9). 9. Glucocorticoids inhibit dendritic cell maturation induced by Toll-like receptor 7 and Toll-like receptor 8. A. Larangé, D. Antonios, M. Pallardy and S. Kerdine-Römer. J Leukoc Biol. 2012 Jan;91(1):105-17. 10. Evidences for Chemical and Cellular Reactivities of the Formaldehyde Releaser Bronopol Independent of Formaldehyde Release. Kireche M, Peiffer JL, Antonios D, Fabre I, Giménez-Arnau E, Pallardy M, Lepoittevin JP, Ourlin JC. Chem Res Toxicol. 2011 Oct 28. 11. Mechanisms of IL-12 synthesis by human dendritic cells treated with the chemical sensitizer NiSO4. Antonios D, Rousseau P, Larangé A, Kerdine-Römer S, Pallardy M. J Immunol. 2010 Jul 1;185(1):89-98. 12. Deoxynivalenol (DON) levels in food in Lebanon and validation of a HPLC/UV detection for the determination of DON in crushed wheat (bulgur). D.Antonios, V.Guitton, S. Darrozes M.Pallardy and H Azouri. Food Additives and Contaminants: Part B, Volume 3, Issue 1 March 2010 , pages 45 – 51 13. Acetaminophen and lipopolysaccharide act in synergy for the production of pro-inflammatory cytokines in murine RAW 264.7 Macrophages. S.Lacour, D.Antonios, J-C Gautier and M.Pallardy. J Immunotoxicol. 2009 Jun;6(2):84-93. 14. TLR7 and TLR8 agonists trigger different signaling pathways for human dendritic cells maturation. A. Larangé, D. Antonios, M. Pallardy, S.Kerdine-Romer. J Leukoc Biol. 2009 Apr;85(4):673-83. 15. Metallic haptens induce differential phenotype of human dendritic cells through activation of mitogen activated protein kinase and NF-κB pathways. Antonios D, Ade N, Kerdine-Römer S, Assaf-Vandecasteele H, Larangé A, Azouri H, Pallardy M. Toxicol in vitro 2009 Mar;23(2):227-234. 16. NF-kappa B plays a major role in the maturation of human dendritic cells induced by NiSO(4) but not by DNCB. N. Ade, D. Antonios, S. Kerdine-Romer, F. Boisleve, F. Rousset and M.Pallardy. Toxicol Sci. 2007 Oct;99(2):488-501. Published abstracts : 17 Hypersensitivity Reactions To Chemotherapy Agents In Lebanon: A Retrospective Epidemiological Analysis From 2015 to 2023 at a Tertiary Center. Carla Irani , Tarek Nahle , Ghiwa Abou Jaoude , Diane Antonios , Hayat Azouri. Publication Abstract dans le “Journal of Allergy and Clinical Immunology”2024. 18 “An enhanced technique exploring the role of T cells in drug allergies” Irani C, Dagher J, Antonios D, Pallardy M, Azouri H. Publication Abstract dans le “Journal of Allergy and Clinical Immunology”, Février 2021 (https://www.jacionline.org/article/S0091-6749(20)31844-3/abstract). 19 Updates in Nickel Allergy: In Vitro Regulation of Interleukin-12 Cytokine Family in Human Dendritic Cells and Identification of Naive T-cells Specific to Nickel. R Bechara, D Antonios, M Azoury, H Azouri, M Pallardy. International journal of Toxicology 36 (1), 62-63. 2017. 20 Nickel sulfate regulates IL-12 cytokine family in human dendritic cells impacting T-cell polarization: Novel role for Jak-STAT and NFIL-3 pathways. M Pallardy, R Bechara, D Antonios, H Azouri. Toxicology Letters, Volume 280, Supplement 1, 20 October 2017, Page s84. 21 Nickel sulfate and Interleukine-12 cytokine family: Shedding light on Interleukine-27 R. Bechara, S. Berrabah, D. Antonios, H. Azouri, M. Pallardy. Toxicology Letters, Volume 258, Supplement, 16 September 2016,Page s230 22 The chemical sensitizer nickel sulfate triggers the production of interleukin(IL)-23 by human dendritic cells: Involvement of p38MAPK and NF-κB pathways. Toxicology Letters, Volume 238, Issue 2, Supplement, 16 October 2015, Page s224. R. Bechara, D. Antonios, H. Azouri, M. Pallardy. 23 Signal transduction induced by small chemicals in human dendritic cells. Toxicology Letters, Volume 189, Supplement, 13 September 2009, Page s170. Saadia Kerdine-Römer, D. Antonios, Alexandre Larangé, Philippe Rousseau,M. Pallardy. 24. JNK inhibition by glucocorticoids prevents the maturation of dendritic cells induced by Toll-like receptor 7 and Toll-like receptor 8. A Larange, D Antonios, M Pallardy, S Kerdine-Romer. The FASEB Journal 22.2008. (2_supplement), 386-386 . 25. Metallic haptens induce dendritic cells activation. S Kerdine-Romer, D Antonios, A Larange, C Espinasse, M Pallardy. Toxicology letters 158, S92-S92. 2005.

• Prix du meilleur poster lors de la réunion annuelle de la société française d’immunologie
• Prix du meilleur article de l’année 2010 dans le domaine de l’immunotoxicologie (SOT – Mars 2011 – Washington)